Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 2nd International Conference on Infection Control and Prevention Chicago, Illinois, USA.

Day 2 :

OMICS International Infection Control  2017 International Conference Keynote Speaker Lia Monica Junie photo
Biography:

 L M Junie is a Professor, Head Microbiology Department in Iuliu Hatieganu University of Medicine and Pharmacy, Cluj Napoca, Romania. She is a Coordinator of Resident Doctor’s in the Laboratory Medicine specialty and Leadership PhD Doctor’s thesis in Medicine field. She is a Board Member of European professional Societies ESCMID (ESGCP Study Groups), Society of Chemotherapy, Scientia Parasitologica Pro Vita and is a Reviewer of international reviews, Member of International organizations, Director/Coordinator in research projects. She has more than 63 papers published in full in international journals and is an Editorial Board Member of national reviews. She is an Organizer/President, Keynote, Invited Speaker and Chair of International and National Congresses.

Abstract:

Background: Staphylococcus aureus infections remain a serious medical problem and prompt initiation of appropriate antimicrobial chemotherapy is important to improve the prognosis of these infections.
Methods: All cultures were incubated into the BacT/alert (Biomérieux) blood culture system. Species identification was
achieved by standard procedures and by the automated system Vitek 2 (Biomérieux). The susceptibility to antibiotics was performed using the disk diffusion method (Kirby-Bauer) according to the CLSI criteria and by Vitek 2 System, for a total of 1268 strains (855 in Greece and 248 in Romania).
Results: From 6494 blood cultures sets, positive cultures were 855 (13,2) and 51 of them (5,9%) yielded Staphylococcus aureus.
34.3% of S. aureus isolated strains were MRSA in Romania. 47.1% of the S. aureus tested strains were resistant to clindamycin
in Ro. All the S. aureus strains tested to glycopeptides antibiotics and the second line antistaphylococcal antibiotics: linezolid,quinupristin/dalfopristin was found sensitive. 16.7% of all strains were resistant to gentamicin in Ro. Moderately increased
percentages of resistance were found by testing the strains to ciprofloxacin in Ro (20.3%). Low levels of resistance were
observed by testing the strains to trimethoprim/sulfamethoxazole (3.9%) in Ro. All the S. aureus strains tested were sensitive
to all antibiotics in 2016.
Conclusion: The majorities of the isolated strains were MSSA and exhibited higher % susceptibility, in contrast to those that
isolated in 2016. Therefore, the policy that was adapted to avoid the spread of resistant strains was effective and recommended
the need to introduce the control program in Romania.

Break: 10:40-11:00
  • Microbial Infections | Infection Control and Hospital Epidemiology | Medical Microbiology | Immunity and Vaccination | Antimicrobial Resistance | Pediatric Infections Prevention and Control
Speaker

Chair

Lia Monica Junie

Iuliu Hatieganu University of Medicine and Pharmacy, Romania

Session Introduction

Narveen Jandu

Gannon University, USA

Title: Application of online tools & resources on foodborne outbreaks for microbiology instruction

Time : 11:45-12:10

Biography:

Dr. Narveen Jandu is currently working as an assistant professor in the Gannon University, Pennsylvania, USA.

Abstract:

In recent years, there have been several newsworthy outbreaks of infectious disease agents, such as Zika, Ebola and the 2015 Escherichia coli O26 outbreak associated with Chipotle restaurants in the United States. During these outbreaks, numerous resources can provide the public, faculty and students with timely, accurate, and credible information. The outbreaks associated
with these microbiological infectious disease agents can and should be incorporated into ongoing microbiology courses. One way to do this is to utilize the excellent resources available from the Centers for Disease Control and Prevention (CDC). The CDC website provides a wealth of resources and information on numerous current, ongoing and past outbreaks. Understandably, the CDC website can be overwhelming for new users, such as students. Students, however, can be gradually introduced to some of these resources
through planned assessments that will allow students to learn both microbiology content and how to utilize online resources. Two specific online resources were selected for students to develop their understanding of foodborne outbreaks. These resources include the FOOD (Foodborne Outbreak Online Database) Tool and the Multistate Foodborne Outbreaks webpage, which are both available through the CDC website. Both resources allow users to retrieve up-to-date information on ongoing outbreaks, which allows the
content in a microbiology course to be relatable to current news events. As well, students can utilize these resources to learn how to access public data, use spreadsheets, generate charts, and perform quantitative data analysis and interpretation. For teaching purposes, these resources are valuable for professors to generate figures and graphics for lectures. The utilization of real-time online data on ongoing outbreaks on infectious disease agents provides a more relatable and application based approach to understanding
how infectious disease agents cause foodborne and other outbreaks.

Speaker
Biography:

Pavithra Saikumar is a Visiting Scholar in Genetics and Stem Cell Laboratory at University of Pacific, San Francisco, where she is currently researching the effect of folic acid in ameliorating hypoxia induced stem cell changes, and its correlation to non-syndromic craniofacial cleft lip and palate. She has obtained her Medical degree from India, at the culmination of which she was awarded the “Best Outgoing Student” for her academic excellence and research interests. She has worked in Department of Internal Medicine, where she treated patients and organized medical camps in rural and underserved areas. She plans to continue her research and provide healthcare as a physician in the US.

Abstract:

Intestinal parasitic infection is one of the major health problems in many developing countries, including India. Intestinal protozoans and helminths are widely prevalent and cause significant medical and public health problems. The study described in this poster was conducted to determine the prevalence of intestinal parasitic infections in the age group of 8-12 years and correlate their growth parameters to parasitic infections. 500 students participated in the study, detailed history and physical examination was done and stool samples were collected. In our study, out of 500 samples, 280 were positive for intestinal helminths. Among 280 positive samples, 180 (64.3%) were boys and 100 (35.2%) were girls. Furthermore, out of 280 positive samples, 110 (39.2%) were positive for Ascaris lumbricoides, 80 (28.5%) for Ancylostoma duodenal, 40 (14.2%) for Strongyloides stercoralis, 27 (9.6%) for Hymenolepis nana and 23 (8.2%) for Enterobius vermicularis. In our study, 40.3% of infested children were underweight and 29.6% of infested children were short stature. The results from our study indicate that parasitic infections are more common in male children and these infections had a drastic effect on growth of these children. Appropriate diagnosis, effective health education, prompt treatment, regular deworming and better sanitary measures will reduce the parasitic infections and improve the health status of children.

Speaker
Biography:

Abbas Ali HUSSEINI completed his Ph.D at Ankara University. His research interests lie in the area of infectious disease. He has researched on epidemiology and molecular profile of HIV, HTLV, HBV, HCV and HDV. Abbas has experience of designing different NA isolation kits and HBV, HCV, HIV and CMV quantitative real time PCR Kits.

Abstract:

Background: Infections caused by HBV, HCV and HDV are global public health problem particularly in low-income countries such as Afghanistan (1). Although limited studies on these diseases provide important information about seroepidemiology of viral hepatitis in this country but molecular epidemiology of these viruses has also remain completely vague in Afghanistan. This study
give a clue about HBV, HCV and HDV genotypes, sub genotypes and subtypes circulating among general population of Afghanistan
and also HBV vaccine scape and Anti-viral resistance mutation prevalence as well.
Methodology: Totally 234 HBsAg, 44 anti-HCV and 5 Anti-Delta positive Samples sera belong to age group 25-70 years old of permanent residues of five region of Afghanistan were included in this study.After quantify viral load by real time PCR, HBV, HCV and HDV genotyping were performed by amplifying and direct sequencing of a segment of the HBV Pre S1, HCV NS5B, and HDV Delta antigen regions, from 61 HBV, 29 HCV and 1 HDV samples containing sufficient amounts of genetic material. In addition, to
HBV serotyping, HBV vaccine escape and anti-viral resistance mutations analysis a segment of related S gene (coding for aa100-167) and HBV polymerase region (coding for aa 160–257) of 20 patient were amplified and sequenced respectively.
Results: All HBV isolate were dispersed throughout the genotype D branch, 60 samples subgenotype D1 and one sample in subgenotype D4 and ayw2 was the only subtype found in Afghan patients with HBsAg positive. throughout the vaccine escape and antiviral resistance mutation analysis, one mutation in P120S and Q215P positions in the respective S gene and in the HBV
polymerase region, respectively, were detected. The Anti-HDV prevalence among HBsAg positive patient in our study was 2.5% and the single sample of HDV we were able to amplified and sequenced partially was stayed in Branch genotype I in phylogenetic analysis. Nucleotide sequence analysis of HCV isolates has shown subtype HCV-1b in 22 (75.86%), HCV-3a in 6 (20.69%) and HCV-3b in 1 (3.44%) patients.
Conclusion: Finally, it can be said that there is little genotypic variation in Afghan patients. HBV genotype D1, subtype ayw 2, HDV RNA type 1, and HCV RNA genotype 1b are likely to be dominant in Afghan patients. We recommend further study in this subject.

Speaker
Biography:

Dhruv Mamtora has completed his MBBS in 2010 from Lokmanya Tilak Municipal Medical College, Mumbai, India. He also did his MD in Medical Microbiology from Government Medical College, Miraj, Maharashtra, India in the year 2013. He is the Head of Microbiology and Infection Control Department at S L Raheja Hospital, a 150 bedded multispecialty hospital and center of excellence for diabetes and oncology. He has published papers in journals and has been serving as reviewer for Pubmed indexed journal. He is also Media Subject Expert on infection control, microbiology, antimicrobials and outbreaks.

Abstract:

Diabetes is a slowly progressing disease of modern India. India has the highest number of diabetes patients amongst WHO member countries. Around 15% of patients with diabetes develop lower limb ulcers. Diabetes is the commonest cause of non-traumatic amputations due to chronic polymicrobial infections of foot and lower limb. Hence a study was conducted to further elucidate the
details about lower limb infections. Around 1294 isolates were studied from 758 patients in year 2016. Four hundred and sixty seven cultures showed no growth. Four hundred and twenty cultures showed single isolate, 247 cultures grew two isolates, 91 cultures grew three or more organisms. Colonization pattern was as follows: Candida species were 30, coagulase negative Staphylococcus (CoNS) were 28, Aspergillus species seven and two Mycobacterium which were seen only on smear examination. Most common were bacterial infections, followed by fungal infections. The bacteria most commonly isolated were Staphylocccus species (267), Pseudomonas
aeruginosa (212), Klebsiella species (196), E. coli (160), proteus/providential/morganella group (151) followed by Streptococcus species
(138) comprising of 88 enterococci and 38 streptocci. Multidrug resistant organisms were MRSA (62), carbapenem resistance in Pseudomonas aeruginosa (33.5%), Klebsiella pneumoniae (34.5%), E. coli (15.29), proteus group (6.76%) and Vancomycin resistant Staphylococcus aureus (VRSA) 1 isolate. One Enterococcus raffinosus was isolated which was resistant to vancomycin. Certain relatively rare organisms viz. Burkholderia species (3), Alkaligenes group (1), Sphingomonas spp. (4) and Cryptococcus laurentii (3)
and Raoultella ornitholytica (2). In context of emergence of carbapenem resistant gram negative isolates, delayed wound healing, immunocompromised status of Type II diabetes patients; the only option left for the diabetic foot surgeons will be amputation, if the emergence of antimicrobial resistance is not addressed appropriately. Timely treatment is of utmost importance as time is tissue.

Barley Chironda

Toronto East General hospital, Canada

Title: Implementation science a missing ingredient in healthcare surface disinfection

Time : 14:25-14:50

Speaker
Biography:

Barley Chironda is working as a Nurse and is the Social Media Manager of IPAC Canada. He is also the past President of IPAC-GTA. He is also the National Healthcare Sales Director and Infection Control Specialist with Clorox Canada. He is certified in Infection Prevention and Control (CIC™) and has worked extensively in Infection Control. He has been an integral force behind the successful decline in Clostridium difficile infections by implementing innovative technology and quality improvement behavioural change. He has also volunteered as the Infection Control Specialist with the Cdiff Foundation.

Abstract:

Despite numerous interventions in surface disinfection, healthcare facilities continue to have surfaces that are not adequately cleaned/disinfected. The author, in this presentation will go through the latest literature, adressing some of the reasons as to why this is the case. The talk will also provide insights into how, after considering all the key criteria of selcting surface disinfenctants the
missing link seems to be the implementation science. Key comparisons will be drawn from other areas of infection control such as hand hygiene, surgical checklists, etc. where the failure of having proper implementation has led to poor clinical outcomes. The talk will focus on implementation science and product rollout in key areas such as electronic health records and learning management system. Therefore it will be showcasing the efficacy of implementation science in the realm of surface disinfenctants. This presentation
will put some light on the microbiological adavancements in surface disinfection and how these still yield to proper implementation.

Al dubisi Fatimah

University of Toronto, Canada

Title: Invasive streptococcal Pyogenes infection

Time : 14:50-15:15

Speaker
Biography:

Al dubisi Fatimah has completed her Pediatric Infectious Board Certificate in Saudi Arabia 2014, She used to work as a Staff in the Division of Infectious Diseases and later as a Director of Infection Control Division in the Minsitry of Health, Saudi Arabia 2014-15. Currently she is doing an extensive work on infectious diseases at the Hospital of Sick Childen, University of Toronto since 2016 with extra focus on tuberculosis. She has couple of case reports and a published study about Clostridium difficile. She is currently working on some reasearch projects in the tuberculosis and antibiotic stewardship

Abstract:

Whenever Streptococcus pyogenes infection comes into attention, most people would simply think of strept pharyngitis, what they used to call for a long time a “strep throat”. In few other occasions, this might indicate a localized skin infection that has been traditionally treated with a few-day course of antibiotics. Unfortunately, this is not always the case. Invasive streptococcal infection
has been increasingly reported in the recent years. The spectrum of the disease ranges from soft tissue infection to severe sepsis, toxic shock syndrome and even death. Not uncommon, post streptococcal sequelae have been irreversible and led to permanent disabilities even when the best antibiotics have been used. The strain characteristics and the genetic profiles of the isolates, the so called "super antigens" are responsible for the different clinical presentations and most importantly the seriousness of the disease. We here present a case of an 11 year old healthy girl who developed severe streptococcal sepsis that was complicated with toxic shock syndrome, multi-organ dysfunction and purpura fulminans. Over the course of her illness, she developed cardiac asystole and sever vascular compromise that have unfortunately led to the amputation of two limbs. And yet she had to undergo a long-term rehabilitation program that could last for years.

  • Workshop

Session Introduction

Guy Hagues Fontaine

HDR Hôpital de la Salpêtrière, France

Title: Superimposed myocarditis can lead ARVD patients to acute progression to CHF
Biography:

G H Fontaine has made 15 original contributions at the inception of cardiac pacemakers in the mid-60s. He has identified ARVD as a side work during the beginning of antiarrhythmic surgery in the late 70s. He has published more than 860 scientific papers including 197 book chapters. He was the Reviewer of 17 journals both in clinical and basic Science. He has also served for 5 years as a Member of the Editorial Board of Circulation. He has been invited to give 11 Master’s lectures of 90 minutes each during three weeks in the top universities of China (2014).

Abstract:

ARVD is one of the cause of sudden death in the young and the athlete leading to the crucial problem of indication of ICD implantation. However, the occurrence of an acute phenomenon of superimposed myocarditis may be in some cases a major problem leading to acute devastating CHF. Here, we report such a case in whom the clinical course was illustrated by release of troponin and progressive decrease in LVEF and finally heart transplant confirming the diagnosis of ARVD but also showing a typical involvement of both ventricles by histological signs of myocarditis. A male teenager with negative family
history presented one episode of palpitation after a game of soccer. Four months later, he had a documented episode of VT with LBBB. Echo showed major dilatation of right atrium associated with RV dilatation, LV size and function were normal. SAECG was positive and contrast angiographies were typical of ARVD. VT was not inducible at EPS but a TdP-like pattern followed by LBBB VT was observed after Isoproterenol injection. CMR confirmed RV dilatation and thinness of the free wall, also diffuse RV hypersignal. Some days later the patient had chest pain, increase in troponin level and T waves changes in inferior leads. Viral test was positive for para-Influenzas III. Two months later Frequent PVCs. Three months later non-sustained episodes of VT. Same positive viral testing. FEVG was 57%. Abrupt change in disease progression was observed three months later. Severe VT occurred during exercise necessitating DC Shock. Major Increase in Troponin level reached 120 ng/ml. ICD was implanted. After nine months multiple episodes of VT: LBBB + superior axis was associated with sign of RV failure. Major dilatation of RV and decrease in LVEF 45% occurred as well as signs of hepatic dysfunction. Few months later, CHF was associated with LVEF 25%. Clots in the LV were observed with probable coronary emboli. Successful heart transplantation was performed. Pathology showed in RV evidence of typical ARVD with clusters of lymphocytes. The same pattern was observed in the LV with multiple foci of lymphocytes, associated with areas of myocardial necrosis. This case illustrates the recent work of Lopez-Ayala (HR 2016) confirming the suggested association of ARVD with myocarditis which can be based on the same
genetic factor not available in our old case (Dr F Roland, Medical Thesis, University of Toulouse 2002).

  • Video Presentation

Session Introduction

Zbigniew Pawlowski

Poznan University of Medical Sciences, Poland

Title: Taeniasis/neurocysticercosis control: Medical aspects

Time : 15:15-15:40

Speaker
Biography:

Zbigniew Pawlowski is a Professor Emeritus of the Medical University in Poznan, Poland. He was a Chief of the Clinic of Parasitic and Tropical Medicine in Poznan (1970-1979 and 1987-1996) and an Organizer of the Intestinal Parasitic Infection Unit at World Health Organization in Geneva (1979-1986). He was active in several international parasitic and tropical medicine institutions (long term WHO expert, honorary member of several professional societies incl. American Society of Tropical Medicine and Hygiene). He has a DTMH diploma of the Liverpool School of Tropical Medicine (1964) and was for years involved in the Association of the Directors of the European Schools of Tropical Medicine. For almost three decades, he collaborated scientifically with the Centre for Disease Control in Atlanta, US. In 1999, he was awarded Doctor Honoris Causa by Warsaw Medical University.

 

Abstract:

Neurocysticercosis due to a larval stage of Taenia solium is a serious public health problem across low and middle-income countries of Latin America, Sub-Saharan Africa and Asia. It is a considerable problem in Europe and the United States, caused by immigration of T. solium carriers from endemic countries. Neurocysticercosis has a various clinical presentation, which ranges from asymptomatic infections to fatal cases. The most common symptoms are a late-onset epilepsy or partial seizures. The control of neurocysticercosis considers two aspects of T. solium transmission; it may be an indirect meat-born zoonotic control measure preventing human taeniosis by limitation of cysticercosis in pigs and a direct control measure by eradication of human tapeworm carriers, being the sole faecal-borne source of cysticercosis for humans and pigs. The control measures can be realized by the organized local or regional eradication programs as well as the routine daily medical and veterinary services. There is much progress in the veterinary control measures in endemic areas. However, the medical control of neurocysticercosis is still inadequately realized in spite of the improvements in diagnosis (fecal antigen tests), new taenicides (niclosamide, praziquantel) and better understood epidemiology (focality of transmission) of human T. solium taeniosis/cysticercosis. Several rules are proposed to be accepted in the medical control measures: Leaving untreated any detected case of T. solium taeniosis, spreading neurocysticercosis, is a medical error, due to difficulties in T. ,solium diagnosis the specific anthelminthic treatment has to be considered also in a suspected case of taeniosis (eg., there is a potential carrier of a T. solium tapeworm in every fourth case of neurocysticercosis), any Taenia tapeworm infection (T. solium, T. saginata, T. asiatica) deserves a specific treatment in order that T. solium is not missed, modern or traditional taenicides has to be easily available where needed, possibly free of charge, instruction has to be elaborated how to avoid the neurologic adverse reactions to praziquantel and preserve the stability and efficacy of niclosamide, a professional training in the control of taeniosis/neurocysticercosis has to be intensified in endemic areas, especially among mental health, public health and primary health care services as well as among veterinarians preventive screening of the T. solium tapeworm infected immigrants or visitors to endemic countries as well as local street meat vendors and specific treatment of those with a positive fecal antigen test, have to be considered. 

Biography:

Abdelhakam Hassan is a Technologist and is working as a Lecturer of Medical Microbiology (bacteriology, mycology, virology and molecular biology) who rapidly learnt all the aspects related to his study. He has more than four years' experiences in the field of drug resistance. He has done his MSc complementary research in the extended spectrum β-lactamase in K.pneumoniae (ESBL).

Abstract:

Background: Antibiotic non susceptible Bacteria have been increased and disseminated all over the world. So this resistant strain with treatment difficulty may threat the Patients lives. However the last line of treatment for such infection is antibiotic from Carbapenem family (1). One of the most virulence bacteria with pandrug resistant is Klebsiella pneumoniae  which associated with high associated with morbidity and mortality (2). In this study, the phenotypic and genotypic features of carbapenem-resistant K.pneumoniae strains isolated in some Sudanese hospitals were studded.

Methodology: Strains of K. pneumoniae resistant to at least one carbapenem (imipenem or meropenem) were collected from May, 2015 to January, 2017. Isolates were obtained from clinical samples of patients treated inside the hospitals. Carbapenem resistance was confirmed using modified Hodge test (MHT) as phenotypic test and real-time PCR for genotypic detection.

Findings: A total 96 strains of K. pneumoniae of different non duplicated isolates were obtained from following samples; urine (47), wound swab (22), sputum (16), stool (3), CSF (1), HVS (4), seminal fluid (1), peritoneal (1) and blood.

All these isolates were found to be resistant to carbapenem by disc sensitivity test and modified Hodge test (MHT) revealed 63 (65.6%) isolates were carbapenemase producers. Seventy-two percent (70/96) isolates were positive for carbapenemase genes; 59.4% (57/96) were positive for blaKPC genes, 57.3% (55/96) were positive for blaNDM genes, 37.5% (36/96) were positive for blaVIM genes and 35.4% (34/96) were positive for blaOXA-48 genes. Nineteen isolates possessed four genes (blaKPC, blaNDM, blaVIM and blaOXA-48) , fourteen isolates possessed three genes{(blaNDM, blaVIM and blaOXA-48=6), (blaKPC, blaNDM, and blaOXA-48=3), (blaKPC, blaNDM and blaVIM =3), (blaKPC, blaVIM and blaOXA-48=2)}, 27 isolates possessed two genes{(blaKPC and blaNDM =21), (blaKPC, blaOXA-48=2), (blaNDM and blaVIM=3), (blaNDM and blaOXA-48=1)}, 10 isolates possessed only one gene (blaKPC=8, blaOXA-48=1 and blaVIM =1) and the remaining 26 isolates were free from these genes.

Conclusion & Recommendation: In Sudan, the most common type of carbapenemase gene multidrug-resistant K. pneumoniae is KPC. Co-production of KPC, VIM, NDM and OXA-48 genes are found in K. pneumoniae. To our knowledge, this study was done for the first time in Sudan. Therefore, it is necessary to determine carbapenem resistance in K.pneumoniae isolates and take essential infection control precautions to avoid spread of this resistance.